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Representative western blots of phosphorylated (p) and total (t) forms of proteins involved in the upregulation of protein synthesis (A) and least squares mean pooled SE expression of phosphorylated forms of those proteins (Akt at Ser473, rpS6 at Ser235/236 and Ser240/244, and 4E-BP1 at Thr 37/46; B) in gluteal muscle samples obtained from control horses (n = 6; white bars) and horses with PPID (6; black bars) after feeding. Expression of the phosphorylated forms of proteins was expressed relative to total expression, and the values for control horses were expressed as an arbitrary value of 1.0.
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Representative western blots of phosphorylated (p) and total (t) forms of NF-κB p65 (an indicator of inflammatory status; A) and least squares mean pooled SE expression of the phosphorylated form of that protein (NF-κB p65 at Ser536; B) in gluteal muscle samples obtained from control horses (n = 6; white bars) and horses with PPID (6; black bars) after feeding. Expression of the phosphorylated form of the protein was expressed relative to total expression, and the values for control horses were expressed as an arbitrary value of 1.0.
Representative western blots of proteins involved in negative regulation of protein synthesis (AMPKα and myostatin; A) and least squares mean pooled SE expression of the phosphorylated form (AMPKα at Thr172) or relative abundance (myostatin; B) of those proteins in gluteal muscle samples obtained from control horses (n = 6; white bars) and horses with PPID (6; black bars) after feeding. Expression of the phosphorylated form (p) of AMPKα was expressed relative to total expression (t), expression of myostatin was expressed relative to α-tubulin expression, and the values for control horses were expressed as an arbitrary value of 1.0.
Representative western blots of FoxO1, MuRF1, and atrogin-1 (A) and least squares mean pooled SE expression of the phosphorylated form (FoxO1) or relative abundance (MuRF1 and atrogin-1; B) of those proteins in gluteal muscle samples obtained from control horses (n = 6; white bars) and horses with PPID (6; black bars) after feeding. Expression of the phosphorylated form (p) of FoxO1 was expressed relative to total expression (t), expression of MuRF1 and atrogin-1 was expressed relative to α-tubulin expression, and the values for control horses were expressed as an arbitrary value of 1.0. 2ff7e9595c
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